then the expected genome size range is: 50% to 150% of median.if 4 standard-deviations is more than 50% of the median,.then the expected genome size range is: 80% to 120% of median.if 4 standard-deviations is less than 20% of the median,.then the expected genome size range is: median - 4x std-dev to median + 4x std-dev.if 4 standard-deviations is between 20% and 50% of the median,.calculate the median and standard-deviation (std-dev) of the genome assembly sizes.if the species has at least four genome assemblies remaining.

skip assemblies that are flagged as partial, anomalous or excluded-from-RefSeq for other reasons and any others whose genome size is radically different from others of the species, as calculated by an interquartile range (IQR) method.The rules used to calculate the expected genome size range for a species can be summarized as: The expected genome size for eukaryotes is the value for a haploid genome assembly. gaps and runs of 10 or more Ns are ignored. The 'genome size' is the ungapped-length of the genome assembly, i.e. NBCI calculates an expected genome size range for all species that have at least four assemblies in the NCBI Assembly database. The NCBI Genome Size Check API can be used to check the size of a genome assembly against the expected genome size range in advance of submission. gross contamination with other sequences.targeted sub-genome assembly not flagged as partial genome representation.metagenome submitted as an organism genome.GenBank compares the size of a submitted genome assembly to the expected genome size range for the species to identify outliers that can result from errors such as:

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